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Part 1: Document Description
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Citation |
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Title: |
A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE |
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Identification Number: |
doi:10.21979/N9/XKMAEX |
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Distributor: |
DR-NTU (Data) |
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Date of Distribution: |
2022-10-31 |
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Version: |
1 |
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Bibliographic Citation: |
Thibault, Guillaume; Yap, Wei Sheng, 2022, "A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE", https://doi.org/10.21979/N9/XKMAEX, DR-NTU (Data), V1, UNF:6:5KJyBwAUciUBv92jsbfKDg== [fileUNF] |
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Citation |
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Title: |
A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE |
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Identification Number: |
doi:10.21979/N9/XKMAEX |
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Authoring Entity: |
Thibault, Guillaume (Nanyang Technological University) |
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Yap, Wei Sheng (Nanyang Technological University) |
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Software used in Production: |
Image Studio |
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Grant Number: |
under its NRF-NSFC joint research grant call (NRF2018NRFNSFC003SB-006) |
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Distributor: |
DR-NTU (Data) |
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Access Authority: |
Thibault, Guillaume |
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Depositor: |
Thibault, Guillaume |
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Date of Deposit: |
2022-09-26 |
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Holdings Information: |
https://doi.org/10.21979/N9/XKMAEX |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Unfolded protein response, Endoplasmic reticulum stress, PERK, IRE1, Synthetic biology, SCRaMbLE, Yeast, Human |
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Abstract: |
Human proteins expressed in yeast are common to enhance protein production while the expression of functional human pathways remain challenging. Here, we propose a simple and economical high-throughput gene assembly method to create a yeast megaplasmid library from human cDNA to screen for minimal human functional pathways. We introduced artificial promoters followed by symmetric loxP sites into the megaplasmids using the Golden Gate assembly coupled with streptavidin-bead-based purification. The isolated high molecular weight, randomly assembled cDNA megaplismids library may be useful for high-throughput directed evolution experiments and may be adapted for use in other model organisms. |
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Kind of Data: |
Data related to Figure 1 |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Identification Number: |
10.17912/micropub.biology.000657 |
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Bibliographic Citation: |
Yap, WS; Thibault, G (2022). A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE. microPublication Biology. |
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Citation |
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Identification Number: |
10356/165616 |
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Bibliographic Citation: |
Yap, WS; Thibault, G (2022). A new combinatorial megaplasmid library assembly method designed to screen for minimal pathways by using SCRaMbLE. microPublication Biology. |
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File Description--f98220 |
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File: 56bp-GFP compiled.tab |
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Notes: |
UNF:6:5KJyBwAUciUBv92jsbfKDg== |
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List of Variables: |
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Variables |
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f98220 Location: |
Variable Format: character Notes: UNF:6:IteZTGxEYpcTWGq7sXGuVA== |
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f98220 Location: |
Summary Statistics: Min. 34.5; StDev 130.16944085818812; Max. 271.0; Mean 184.16666666666666; Valid 3.0; Variable Format: numeric Notes: UNF:6:4BMpe3esfiYkOUi6kX2IDA== |
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f98220 Location: |
Summary Statistics: Valid 3.0; Max. 714.0; Min. 62.0; Mean 410.0; StDev 328.219438790575 Variable Format: numeric Notes: UNF:6:Tf2Ak8blh6Un7v3UWDVlvw== |
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f98220 Location: |
Summary Statistics: StDev 360.1292592019334; Max. 771.0; Mean 454.1666666666667; Min. 62.5; Valid 3.0; Variable Format: numeric Notes: UNF:6:qtjL9E6L356iGF8BnHzJoQ== |
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f98220 Location: |
Summary Statistics: Valid 0.0; StDev NaN; Max. NaN; Mean NaN; Min. NaN Variable Format: numeric Notes: UNF:6:PnB3/S9m1ongzuanz1s3vw== |
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f98220 Location: |
Summary Statistics: Max. 7.855072463768116; StDev 3.77302727125183; Min. 1.0; Mean 5.338164251207729; Valid 3.0; Variable Format: numeric Notes: UNF:6:Qct0kREl4AHgupwhgGGnbQ== |
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f98220 Location: |
Summary Statistics: StDev 5.293861915977016; Mean 6.612903225806451; Valid 3.0; Min. 1.0; Max. 11.516129032258064 Variable Format: numeric Notes: UNF:6:IgHkyL9qq3aPSkqzhMXt7g== |
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f98220 Location: |
Summary Statistics: Max. 12.336; Mean 7.266666666666667; Min. 1.0; Valid 3.0; StDev 5.762068147230934 Variable Format: numeric Notes: UNF:6:4KmJ1JCFsHEdInrmOterjg== |
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f98220 Location: |
Summary Statistics: Min. NaN; Valid 0.0; Mean NaN; Max. NaN; StDev NaN Variable Format: numeric Notes: UNF:6:PnB3/S9m1ongzuanz1s3vw== |
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f98220 Location: |
Summary Statistics: Mean 1.9378224531274038; Min. 0.0; Max. 2.9736245845757026; Valid 3.0; StDev 1.6795360335639757 Variable Format: numeric Notes: UNF:6:0B7bmbC4jqcWGAZvSfjEKg== |
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f98220 Location: |
Summary Statistics: Min. 0.0; Valid 3.0; StDev 1.8755826204358228; Max. 3.525583953642225; Mean 2.132645376848755 Variable Format: numeric Notes: UNF:6:3ntY9uR9YKmsgyVA/ENnZg== |
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f98220 Location: |
Summary Statistics: Min. 0.0; Max. 3.624802765252947; StDev 1.954874299831048; Mean 2.2353807975682956; Valid 3.0 Variable Format: numeric Notes: UNF:6:6s0WREEq2oqVIBnEoAca1A== |
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Label: |
20211118 Test PaqCI NNNN TCGA (processed).tif |
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Notes: |
image/tiff |
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Label: |
20211125 Golden gate PaqCI (processed).tif |
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Notes: |
image/tiff |
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Label: |
20220321 56bp-GFP (no basal gate).wsp |
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Notes: |
application/octet-stream |
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Label: |
20220321 56bp-GFP (no basal gate).xls |
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Notes: |
application/vnd.ms-excel |
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Label: |
20220321 56bp-GFP.wsp |
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Notes: |
application/octet-stream |
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Label: |
20220321 56bp-GFP.xls |
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Notes: |
application/vnd.ms-excel |
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Label: |
20220615 56bp-GFP (no basal gate).wsp |
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Notes: |
application/octet-stream |
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Label: |
20220615 56bp-GFP (no basal gate).xls |
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Notes: |
application/vnd.ms-excel |
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Label: |
20220615 56bp-GFP.wsp |
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Notes: |
application/octet-stream |
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Label: |
20220615 56bp-GFP.xls |
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Notes: |
application/vnd.ms-excel |
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Label: |
20220616 56bp-GFP (no basal gate).wsp |
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Notes: |
application/octet-stream |
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Label: |
20220616 56bp-GFP (no basal gate).xls |
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Notes: |
application/vnd.ms-excel |
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Label: |
20220616 56bp-GFP.wsp |
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Notes: |
application/octet-stream |
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Label: |
20220616 56bp-GFP.xls |
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Notes: |
application/vnd.ms-excel |
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Label: |
20220616 cDNA 30c vs 20c vs 20c T-1000 (processed).tif |
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Notes: |
image/tiff |
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Label: |
20220621 PaqCI GG vs ctrl v3 (processed).tif |
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Notes: |
image/tiff |
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Label: |
20220715 T3 T7 megaplasmid colony pcr (partial).tif |
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Notes: |
image/tiff |
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Label: |
20220720 plasmid 6 and 31 (processed).tif |
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Notes: |
image/tiff |
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Label: |
minimal promoter-GFP.pdf |
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Notes: |
application/pdf |
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minimal_promoter-GFP.pzfx |
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Notes: |
text/html |
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Label: |
Yeast_neg_001.fcs |
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Notes: |
application/vnd.isac.fcs |
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Yeast_neg_001.fcs |
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Notes: |
application/vnd.isac.fcs |
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Label: |
Yeast_TATA-TSS_002.fcs |
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Notes: |
application/vnd.isac.fcs |
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Yeast_TATA-TSS_002.fcs |
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Notes: |
application/vnd.isac.fcs |
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Yeast_TATA-TSS (56bp)_003.fcs |
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Notes: |
application/vnd.isac.fcs |
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Yeast_UASc-TATA-TSS_003.fcs |
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Notes: |
application/vnd.isac.fcs |
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Label: |
Yeast_UASc-TATA-TSS_003.fcs |
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Notes: |
application/vnd.isac.fcs |
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Label: |
Yeast_YGT1306 (ev)_001.fcs |
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Notes: |
application/vnd.isac.fcs |
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Label: |
Yeast_YGT1711 (UASc)_002.fcs |
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Notes: |
application/vnd.isac.fcs |