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Part 1: Document Description
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Citation |
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Title: |
Microneedle Coupled Epidermal Sensor for Multiplexed Electrochemical Detection of Kidney Disease Biomarkers |
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Identification Number: |
doi:10.21979/N9/Y22WE7 |
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Distributor: |
DR-NTU (Data) |
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Date of Distribution: |
2023-11-01 |
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Version: |
1 |
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Bibliographic Citation: |
Zheng, Lewen; Zhu, Dan Dan; Xiao, Yi; Zheng, Xin Ting; Chen, Peng, 2023, "Microneedle Coupled Epidermal Sensor for Multiplexed Electrochemical Detection of Kidney Disease Biomarkers", https://doi.org/10.21979/N9/Y22WE7, DR-NTU (Data), V1 |
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Citation |
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Title: |
Microneedle Coupled Epidermal Sensor for Multiplexed Electrochemical Detection of Kidney Disease Biomarkers |
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Identification Number: |
doi:10.21979/N9/Y22WE7 |
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Authoring Entity: |
Zheng, Lewen (Nanyang Technological University) |
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Zhu, Dan Dan (Nanyang Technological University) |
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Xiao, Yi (Nanyang Technological University) |
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Zheng, Xin Ting (Agency for Science, Technology and Research (A*STAR)) |
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Chen, Peng (Nanyang Technological University) |
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Software used in Production: |
Graphpad Prism 8 |
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Grant Number: |
RG110/20 |
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Grant Number: |
RT02/20 |
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Grant Number: |
2019-T2-2-004 |
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Grant Number: |
Mini-BEP grant (C211318009) |
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Distributor: |
DR-NTU (Data) |
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Access Authority: |
Zheng, Lewen |
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Depositor: |
Zheng, Lewen |
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Date of Deposit: |
2023-11-01 |
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Holdings Information: |
https://doi.org/10.21979/N9/Y22WE7 |
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Study Scope |
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Keywords: |
Chemistry, Engineering, Medicine, Health and Life Sciences, Chemistry, Engineering, Medicine, Health and Life Sciences, Transdermal detection, Microneedle patch, Skin interstitial fluid, Multiplex electrochemical assays |
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Abstract: |
Early diagnosis of chronic kidney disease (CKD) and constant monitoring to guide optimal intervention is critical to prevent renal failure and other critical diseases. However, the conventional blood tests in hospital are time-consuming and have poor patient compliance. Herein, we demonstrate a real-time, minimally invasive, and self-administrable approach to detect kidney biomarkers in the skin interstitial fluid (ISF) using a polymeric microneedle coupled electrochemical sensor array (MNESA). Microneedles can readily penetrate stratum corneum and quickly extract ISF onto the sensors. Four biomarkers are simultaneously detected to avoid false positive and provide an accurate assessment of kidney functions. Using an artificial skin model, it is shown that MNSEA gives specific and sensitive responses to these kidney biomarkers in physiologically relevant ranges (phosphate: 0.3–1.8 mM, 3.62 μA/mM; uric acid: 50–550 μM, 4.19 nA/μM; creatinine: 50–550 μM, 12.58 nA/μM; urea: 1–16 mM, 44.6 mV/decade). Using a mouse model, we demonstrate that this approach is as reliable as the commercial assays and is feasible to readily monitor the progression of CDK. |
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Kind of Data: |
experimental data |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Bibliographic Citation: |
Zheng, L., Zhu, D., Xiao, Y., Zheng, X., & Chen, P. (2023). Microneedle coupled epidermal sensor for multiplexed electrochemical detection of kidney disease biomarkers. Biosensors and Bioelectronics, 237, 115506. |
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Citation |
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Identification Number: |
10356/170241 |
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Bibliographic Citation: |
Zheng, L., Zhu, D., Xiao, Y., Zheng, X., & Chen, P. (2023). Microneedle coupled epidermal sensor for multiplexed electrochemical detection of kidney disease biomarkers. Biosensors and Bioelectronics, 237, 115506. |
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Characterization of MNESA.jpg |
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Notes: |
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Electrochemical signals of MNESA in PBS solution in response to stepwise addition of analytes.jpg |
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In vitro calibration of MNESA.jpg |
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In vivo mouse experiments.jpg |
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Responses of electrochemical sensors in MNESA when being immersed in a solution with prescribed concentrations of analytes.jpg |
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The stability and reversibility tests.jpg |
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Notes: |
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