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Part 1: Document Description
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Citation |
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Title: |
Replication Data for: Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress |
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Identification Number: |
doi:10.21979/N9/5BJD2Z |
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Distributor: |
DR-NTU (Data) |
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Date of Distribution: |
2020-06-18 |
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Version: |
1 |
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Bibliographic Citation: |
Ho, Nurulain; Yap, Wei Sheng; Xu, Jiaming; Wu, Haoxi; Koh, Jhee Hong; George, Bhawana; Chong, Shu Chen; Taubert, Stefan; Thibault, Guillaume, 2020, "Replication Data for: Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress", https://doi.org/10.21979/N9/5BJD2Z, DR-NTU (Data), V1 |
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Citation |
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Title: |
Replication Data for: Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress |
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Alternative Title: |
Distinct UPR program activated by LBS |
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Identification Number: |
doi:10.21979/N9/5BJD2Z |
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Authoring Entity: |
Ho, Nurulain (Nanyang Technological University) |
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Yap, Wei Sheng (Nanyang Technological University) |
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Xu, Jiaming (University of British Columbia) |
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Wu, Haoxi (Nanyang Technological University) |
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Koh, Jhee Hong (Nanyang Technological University) |
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George, Bhawana (Nanyang Technological University) |
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Chong, Shu Chen (Nanyang Technological University) |
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Taubert, Stefan (University of British Columbia) |
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Thibault, Guillaume (Nanyang Technological University) |
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Software used in Production: |
Microsoft excel |
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Software used in Production: |
Graphpad Prism |
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Grant Number: |
Nanyang Assistant Professorship program |
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Grant Number: |
NRF2018NRFNSFC003SB-006 |
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Grant Number: |
NRF-NSFC |
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Grant Number: |
Research Scholarship (predoctoral fellowship) |
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Grant Number: |
PJT-153199 |
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Grant Number: |
P40 OD010440 |
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Distributor: |
DR-NTU (Data) |
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Access Authority: |
Guillaume Thibault |
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Depositor: |
Yap, Wei Sheng |
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Date of Deposit: |
2020-06-01 |
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Holdings Information: |
https://doi.org/10.21979/N9/5BJD2Z |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Unfolded protein response; lipid bilayer stress; endoplasmic reticulum stress; endoplasmic reticulum sensor Ire1; UPR-transducer; proteotoxic stress; membrane stress; phospholipid perturbation; S. cerevisiae; C. elegans |
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Abstract: |
Membrane integrity at the endoplasmic reticulum (ER) is tightly regulated, and its disturbance is implicated in metabolic diseases. Using an engineered sensor that activates the unfolded protein response (UPR) exclusively when normal ER membrane lipid composition is compromised, we identified pathways beyond lipid metabolism that are necessary to maintain ER integrity in yeast and in C. elegans. To systematically validate yeast mutants that disrupt ER membrane homeostasis, we identified a lipid bilayer stress (LBS) sensor in the UPR transducer protein Ire1, located at the interface of the amphipathic and transmembrane helices. Furthermore, transcriptome and chromatin immunoprecipitation analyses pinpoint the UPR as a broad-spectrum compensatory response wherein LBS and proteotoxic stress deploy divergent transcriptional UPR programs. Together, these findings reveal the UPR program as the sum of two independent stress responses, an insight that could be exploited for future therapeutic intervention. |
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Kind of Data: |
Excel |
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Kind of Data: |
Prism |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Identification Number: |
10.1083/jcb.201909165 |
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Bibliographic Citation: |
Ho, N., Yap, W. S., Xu, J., Wu, H., Koh, J. H., Goh, W. W. B., ... & Thibault, G. (2020). Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress. Journal of Cell Biology, 219(7). |
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Citation |
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Identification Number: |
10356/149106 |
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Bibliographic Citation: |
Ho, N., Yap, W. S., Xu, J., Wu, H., Koh, J. H., Goh, W. W. B., ... & Thibault, G. (2020). Stress sensor Ire1 deploys a divergent transcriptional program in response to lipid bilayer stress. Journal of Cell Biology, 219(7). |
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Figure 1.zip |
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application/zip |
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Figure 2.zip |
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Figure 3.zip |
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Figure 4.zip |
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Figure 5.zip |
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Figure 6.zip |
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Figure S1.zip |
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Figure S2.zip |
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Figure S3.zip |
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Figure S4.zip |
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Figure S5.zip |
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application/zip |