Replication Data for: The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma. (doi:10.21979/N9/EQXCRF)

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Replication Data for: The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma.

doi:10.21979/N9/EQXCRF

DR-NTU (Data)

2017-12-20

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Soncin, Irene; Sheng, Jianpeng; Chen, Qi; Foo, Shihui; Duan, Kaibo; Lum, Josephine; Poidinger, Michael; Zolezzi, Francesca; Karjalainen, Klaus; Ruedl, Christiane, 2017, "Replication Data for: The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma.", https://doi.org/10.21979/N9/EQXCRF, DR-NTU (Data), V1

Replication Data for: The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma.

doi:10.21979/N9/EQXCRF

Soncin, Irene (School of Biological Sciences, Nanyang Techological University, Nanyang Technological University)

Sheng, Jianpeng (School of Biological Sciences, Nanyang Technological University)

Chen, Qi (School of Biological Sciences, Nanyang Technological University)

Foo, Shihui (Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR))

Duan, Kaibo (Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR))

Lum, Josephine (Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR))

Poidinger, Michael (Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR))

Zolezzi, Francesca (Singapore Immunology Network, Agency for Science, Technology and Research (A*STAR))

Karjalainen, Klaus (School of Biological Sciences, Nanyang Technological University)

Ruedl, Christiane (School of Biological Sciences, Nanyang Technological University)

Flow Cytometry files (.fcs) obtained with BD Fortessa 5-laser flow cytometer (BD Bioscience). Data to be analysed using a FlowJo software (TreeStar, Ashland, OR, USA)

Tier2 MOE2014-T2-1-011

Tier2 MOE2016-T2-1-012

DR-NTU (Data)

Christiane Ruedl

Ruedl Christiane

2017-12-20

https://doi.org/10.21979/N9/EQXCRF

Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Tissue-resident macrophages, Self-renewal, Lamina Propria, Colorectal cancer

Cellular Immunology, Cancer Immunology, Ontogeny

Circulating CCR2+ monocytes are crucial for maintaining the adult tissue-resident F4/80hiMHCIIhi macrophage pool in the intestinal lamina propria. Here we show that, a subpopulation of CCR2-independent F4/80hiMHCIIlow macrophages, which are the most abundant F4/80hi cells in neonates, gradually decline in number in adulthood; these macrophages likely represent the fetal contribution to F4/80hi cells. In colon adenomas of ApcMin/+ mice, F4/80hiMHCIIlow macrophages are not only preserved, but become the dominant subpopulation among tumour-resident macrophages during tumour progression. Furthermore, pro-tumoural F4/80hiMHCIIlow and F4/80hiMHCIIhi subpopulations can self-renew in the tumour and maintain their numbers mostly independently of a bone marrow contribution. In summary, the tumour microenvironment creates an isolated niche for tissue-resident macrophages that favours macrophage survival and self-renewal.

Experimental data

10.1038/s41467-018-02834-8

Soncin, I., Sheng, J., Chen, Q., Foo, S., Duan, K., Lum, J., et al. (2018). The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma. Nature Communications, 9, 582-.

10356/89512

Soncin, I., Sheng, J., Chen, Q., Foo, S., Duan, K., Lum, J., et al. (2018). The tumour microenvironment creates a niche for the self-renewal of tumour-promoting macrophages in colon adenoma. Nature Communications, 9, 582-.

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Fig. 1 part 1 - tumour.zip

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The file contains .fcs files of the tumour samples in Fig. 1

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Fig. 1 part 2 - LP.zip

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The file contains the .fcs files of Lamina Propria samples in Fig. 1

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Fig. 2 part 1 - A, B.zip

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The file contains the .fcs files in Fig. 2 panel A and Fig. 2 panel B

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Fig. 2 part 2 - C.zip

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The file contains the .fcs files in Fig. 2 panel C

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Fig. 3 part 1 - A.zip

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The file contains the .fcs files in Fig. 3 panel A

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Fig. 3 part 2 - B.zip

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The file contains the .fcs files in Fig. 3 panel B

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Fig. 3 part 3 - C.zip

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The file contains .fcs files in Fig. 3 panel C

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Fig. 4 part 1.zip

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The file contains .fcs files in Fig. 4 (part 1)

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Fig. 4 part 2.zip

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The file contains .fcs files in Fig. 4 (part 2)

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Fig. 5 part 1 -A.zip

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The file contains .fcs files in Fig. 5 panel A (part 1)

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Fig 5 part 2 - A.zip

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The file contains .fcs files in Fig. 5 panel A (part2)

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Fig. 5 part 3 - B.zip

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The file contains .fcs files in Fig. 5 panel B

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Fig. 6.zip

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The file contains .fcs files in Fig. 6

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Fig. 7.zip

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The file contains .fcs files in Fig. 7

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Suppl. Fig 1.zip

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The file contains .fcs files in Supplementary Fig. 1

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Suppl. Fig 2.zip

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The file contains .fcs files in Supplementary Fig. 2

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