<?xml version="1.0" encoding="UTF-8"?><!--
This work is licensed under a Creative Commons Attribution-Noncommercial 3.0 United States License.
http://creativecommons.org/licenses/by-nc/3.0/us/
--><!--This XML stylesheet is intended to serve as a generic stylesheet for HTML
display of codebooks marked up according to Version 2.0 of the DDI DTD. Please note that
the part navigation that appears just below the title is dependent upon the 'holdings' tag
located at /codeBook/docDscr/citation/holdings. This navigation element allows the user
to selectively view any of the five parts of the DDI document, assuming those parts are
present. The URI attribute of the 'holdings' tag should contain the URL at which the XML
file is accessed on the Web. If that field is not filled in properly, the navigation
will not work correctly.--><!--It's also important to note that this stylesheet doesn't display all elements.
The following elements do note display:
3.2.1 dataItem (locMap)
3.2.1.1 CubeCoord (locMap)
3.2.1.2 physLoc (locMap)
4.2.1 labl (nCubeGrp)
4.2.2 txt (nCubeGrp)
4.2.3 concept (nCubeGrp)
4.2.4 defntn (nCubeGrp)
4.2.5 universe (nCubeGrp)
4.2.6 notes (nCubeGrp)
4.3.18.5 mrow (nCube)
4.3.18.5.1 mi (nCube)
4.4.2 location (nCube)
4.4.3 txt (nCube)
4.4.4 universe (nCube)
4.4.5 imputation (nCube)
4.4.6 security (nCube)
4.4.7 embargo (nCube)
4.4.8 respUnit (nCube)
4.4.9 anlysUnit (nCube)
4.4.10 verStmt (nCube)
4.4.10.1 version (nCube)
4.4.10.2 verResp (nCube)
4.4.10.3 notes (nCube)
4.4.11 purpose (nCube)
4.4.12.1 cohort (nCube)
4.4.12.1.1 range (nCube)
4.4.13 measure (nCube)
4.4.14 notes (nCube)
In addition, many of the attributes of elements have yet to be set to display.
--><!--
On 2004-06-01 I corrected some errors in this stylesheet, as lists of variables and variable
groups weren't linking properly to the detailed views of said variables and variable groups.
On 2005-06-22 I made some minor modifications to the display of variables lists, and enabled
use of the Link tag within catValu.
On 2005-06-23 I enabled use of Link tag within labl and enabled catStat to display repetitions
in catgry.
--><html>
<head>
<META http-equiv="Content-Type" content="text/html; charset=UTF-8">
<title>Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer’s disease</title>
<style type="text/css" media="screen">
body {
background-color: #FFFFFF;
margin-top: 5px;
margin-left: 5px;
margin-right: 5px;
padding-left: 0px;
padding-top: 0px;
padding-right: 0px;
}
tr.h1 {
background: #000000;
color: #FFFFFF;
}
td.h3 {
vertical-align: top;
text-align: right;
font-style: italic;
}
tr.h2 {
background: #CCCCCC;
color: #000000;
}
caption {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 11pt;
font-weight: bold;
}
p {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 11pt;
}
table {
padding-top: 10px;
padding-bottom: 10px;
border: none;
}
th {
text-align: left;
}
dl {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 11pt;
}
td {
vertical-align: top;
}
blockquote {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 11pt;
}
.noindent {padding-left: 0px;}
.small {font-size: 9pt;}
.red {color: #CC0000;}
ol {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 11pt;
}
ul {
font-family: arial, helvetica, geneva, verdana, sans-serif;
list-style: square;
font-size: 11pt;
}
h1 {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 14pt;
}
h2 {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 12pt;
}
h3 {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 11pt;
font-style: bold;
}
h4 {
font-family: arial, helvetica, geneva, verdana, sans-serif;
font-size: 10pt;
}
</style>
</head>
<body bgcolor="#ffffff" lang="en-US">
<h1>Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer’s disease (doi:10.21979/N9/9RCHLK)</h1>
<table border="0" cellpadding="5" cellspacing="0">
<tr>
<td valign="top">
<p>
<b>View:</b>
</p>
</td><td>
<p>
<a href="#1.0">Part 1: Document Description</a>
<br>
<a href="#2.0">Part 2: Study Description</a>
<br>
<a href="#5.0">Part 5: Other
Study-Related Materials</a>
<br>
<a href="">Entire Codebook</a>
</p>
</td>
</tr>
</table>
<table border="0" cellpadding="5" cellspacing="5">
<tr class="h1">
<th align="left" colspan="2">
<p>
<a name="1.0" id="1.0">Document Description</a>
</p>
</th>
</tr>
<tr class="h2">
<th colspan="2">
<p>Citation</p>
</th>
</tr>
<tr>
<td class="h3">
<p>Title:</p>
</td><td>
<p>Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer’s disease</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Identification Number:</p>
</td><td>
<p>doi:10.21979/N9/9RCHLK</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Distributor:</p>
</td><td>
<p>DR-NTU (Data)</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Date of Distribution:</p>
</td><td>
<p>2021-10-06</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Version:</p>
</td><td>
<p>1</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Bibliographic Citation:</p>
</td><td>
<p>Wu, Xiaoting; Saito, Takashi; Saido, Takaomi C.; Barron, Anna M; Ruedl, Christiane, 2021, "Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer’s disease", https://doi.org/10.21979/N9/9RCHLK, DR-NTU (Data), V1</p>
</td>
</tr>
<tr class="h1">
<th colspan="2">
<p>
<a name="2.0">Study Description</a>
</p>
</th>
</tr>
<tr class="h2">
<th colspan="2">
<p>Citation</p>
</th>
</tr>
<tr>
<td class="h3">
<p>Title:</p>
</td><td>
<p>Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer’s disease</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Identification Number:</p>
</td><td>
<p>doi:10.21979/N9/9RCHLK</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Authoring Entity:</p>
</td><td>
<p>Wu, Xiaoting (Nanyang Technological University)</p>
</td>
</tr>
<tr>
<td class="h3"></td><td>
<p>Saito, Takashi (Laboratory for Proteolytic Neuroscience, Riken Center for Brain Science, Wako, Japan)</p>
</td>
</tr>
<tr>
<td class="h3"></td><td>
<p>Saido, Takaomi C. (Laboratory for Proteolytic Neuroscience, Riken Center for Brain Science, Wako, Japan)</p>
</td>
</tr>
<tr>
<td class="h3"></td><td>
<p>Barron, Anna M (Nanyang Technological University)</p>
</td>
</tr>
<tr>
<td class="h3"></td><td>
<p>Ruedl, Christiane (Nanyang Technological University)</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Software used in Production:</p>
</td><td>
<p>Flowjo</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Grant Number:</p>
</td><td>
<p>MOE AcRF Tier 1</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Distributor:</p>
</td><td>
<p>DR-NTU (Data)</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Access Authority:</p>
</td><td>
<p>Ruedl, Christiane</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Depositor:</p>
</td><td>
<p>Ruedl, Christiane</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Date of Deposit:</p>
</td><td>
<p>2021-07-26</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Holdings Information:</p>
</td><td>
<p>https://doi.org/10.21979/N9/9RCHLK</p>
</td>
</tr>
<tr class="h2">
<th colspan="2">
<p>Study Scope</p>
</th>
</tr>
<tr>
<td class="h3">
<p>Keywords:</p>
</td><td>
<p>Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Inducible fate-mapping analysis demonstrates that neither microglia, disease-associated microglia nor border-associated macrophages are replenished by bone marrow-derived cells in Alzheimer’s disease.</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Abstract:</p>
</td><td>
<p>Brain microglia and border-associated macrophages (BAMs) display distinct spatial, developmental, and phenotypic features. Although at steady-state, the origins of distinct brain macrophages are well-documented, the dynamics of their replenishment in neurodegenerative disorders remain elusive, particularly for activated CD11c+ microglia and BAMs. In this study, we conducted a comprehensive fate-mapping analysis of murine microglia and BAMs and their turnover kinetics during Alzheimer’s disease (AD) progression. We used a novel inducible AD mouse model to investigate the contribution of bone marrow cells to the pool of foetal-derived brain macrophages during the development of AD. We demonstrated that microglia remain a remarkably stable embryonic-derived population even during the progression of AD pathology, indicating that neither parenchymal macrophage subpopulation originates from, nor are replenished by, bone marrow (BM)-derived cells. At the border-associated brain regions, bona fide CD206+ BAMs are minimally replaced by BM-derived cells, and their turnover rates are not accelerated by AD. In contrast, all other myeloid cells are swiftly replenished by BM progenitors. This information further elucidates the turnover kinetics of these cells not only at steady-state, but also in neurodegenerative diseases, which is crucial for identifying potential novel therapeutic targets.</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Kind of Data:</p>
</td><td>
<p>Experimental raw data</p>
</td>
</tr>
<tr class="h2">
<th colspan="2">
<p>Methodology and Processing</p>
</th>
</tr>
<tr>
<th colspan="2">
<p>Sources Statement</p>
</th>
</tr>
<tr class="h2">
<th colspan="2">
<p>Data Access</p>
</th>
</tr>
<tr class="h2">
<th colspan="2">
<p>Other Study Description Materials</p>
</th>
</tr>
<tr>
<th colspan="2">
<p>Related Publications</p>
</th>
</tr>
<tr class="h2">
<th colspan="2">
<p>Citation</p>
</th>
</tr>
<tr>
<td class="h3">
<p>Identification Number:</p>
</td><td>
<p>10.7554/eLife.71879</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Bibliographic Citation:</p>
</td><td>
<p>Wu, X., Saito, T., Saido, T. C., Barron, A. M., & Ruedl, C. (2021). Microglia and CD206+ border-associated mouse macrophages maintain their embryonic origin during Alzheimer's disease. eLife, 10, e71879.</p>
</td>
</tr>
(<a href="https://elifesciences.org/articles/71879">external link</a>)
<tr class="h2">
<th colspan="2">
<p>Citation</p>
</th>
</tr>
<tr>
<td class="h3">
<p>Identification Number:</p>
</td><td>
<p>10356/159385</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Bibliographic Citation:</p>
</td><td>
<p>Wu, X., Saito, T., Saido, T. C., Barron, A. M. & Ruedl, C. (2021). Microglia and CD206⁺ border-associated mouse macrophages maintain their embryonic origin during Alzheimer's disease. ELife, 10, e71879-.</p>
</td>
</tr>
(<a href="https://hdl.handle.net/10356/159385">external link</a>)
<tr>
<th align="left" colspan="2">
<p>
<a name="f75028"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.1_figure supplement 1.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75030"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.1_figure supplement 2.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75025"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.1.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75027"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.2 _figure supplement 1.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75024"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.2.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75031"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.3.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75029"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.4_figure supplement 2.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
<tr>
<th align="left" colspan="2">
<p>
<a name="f75026"></a><a name="5.0">Other Study-Related Materials</a>
</p>
</th>
</tr>
<tr>
<td align="right" valign="top">
<p>Label:</p>
</td><td>
<p>Fig.4.zip</p>
</td>
</tr>
<tr>
<td class="h3">
<p>Notes:</p>
</td><td>
<p>application/zip</p>
</td>
</tr>
</table>
</body>
</html>