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Part 1: Document Description
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Citation |
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Title: |
Human PERK rescues unfolded protein response-deficient yeast cells |
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Identification Number: |
doi:10.21979/N9/IGLQOE |
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Distributor: |
DR-NTU (Data) |
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Date of Distribution: |
2022-09-26 |
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Version: |
1 |
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Bibliographic Citation: |
Thibault, Guillaume; Yap, Wei Sheng, 2022, "Human PERK rescues unfolded protein response-deficient yeast cells", https://doi.org/10.21979/N9/IGLQOE, DR-NTU (Data), V1 |
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Citation |
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Title: |
Human PERK rescues unfolded protein response-deficient yeast cells |
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Identification Number: |
doi:10.21979/N9/IGLQOE |
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Authoring Entity: |
Thibault, Guillaume (Nanyang Technological University) |
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Yap, Wei Sheng (Nanyang Technological University) |
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Software used in Production: |
Image Studio |
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Grant Number: |
NRF-NSFC joint research grant call (NRF2018NRFNSFC003SB-006) |
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Distributor: |
DR-NTU (Data) |
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Access Authority: |
Thibault, Guillaume |
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Depositor: |
Thibault, Guillaume |
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Date of Deposit: |
2022-09-26 |
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Holdings Information: |
https://doi.org/10.21979/N9/IGLQOE |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Unfolded protein response, Endoplasmic reticulum stress, IRE1, PERK, Synthetic biology, Yeast, Human |
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Abstract: |
Protein folding and quality control is tightly regulated at the endoplasmic reticulum (ER), and its disruption is associated with many diseases. In eukaryotes, the accumulation of unfolded protein in the ER is sensed by the three sensors, IRE1, PERK, and ATF6 to activate the unfolded protein response (UPR) to restore ER homeostasis. However, uncoupling the sensing of each sensor and their respective downstream pathways has been challenging as the absence of one is compensated by the remaining two sensors. Here, we report a fully functional human PERK (hPERK) chimeric protein expressed in Saccharomyces cerevisiae that could be used for high throughput screen to identify new PERK inhibitory or activating compounds as well as to characterize the PERK stress sensing mechanisms. |
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Kind of Data: |
Data related to Figure 1 |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Identification Number: |
10.17912/micropub.biology.000592 |
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Bibliographic Citation: |
Yap, W. S., & Thibault, G. (2022). Human PERK rescues unfolded protein response-deficient yeast cells. microPublication Biology, 2022. |
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Citation |
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Identification Number: |
10356/161984 |
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Bibliographic Citation: |
Yap, W. S. & Thibault, G. (2022). Human PERK rescues unfolded protein response-deficient yeast cells. MicroPublication Biology, 2022 |
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Label: |
20190725 TunicamycinTreatment_PERK-GCN4pr-GFP.tif |
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Notes: |
image/tiff |
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Label: |
20190822_PERK_GCN4prHAC1i023.tif |
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Notes: |
image/tiff |
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Label: |
20190912_Chimeric_PERK(LD)_IRE1(CD)001.tif |
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Notes: |
image/tiff |
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Label: |
20191102_Tm_washout_UT_ 1hrTm.0_ 15_ 30_ 60_ 120m washout. rat ctrls_probe_phosphorylated_PERK_eIF2a.tif |
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Notes: |
image/tiff |
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20191104_QuickMicrosomeIsolation_PERK3XHA_TSP_TUB1_SEC61_ctrl.tif |
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Notes: |
image/tiff |
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Label: |
PERK HAC1i carrier.tif |
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Notes: |
image/tiff |
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Label: |
PERK HAC1i Tm.tif |
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Notes: |
image/tiff |
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Label: |
VEC vs hPERK Sui2-P.tif |
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Notes: |
image/tiff |
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Label: |
VEC vs hPERK Tub1.tif |
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Notes: |
image/tiff |