Replication Data for: Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization (doi:10.21979/N9/EX7HL5)

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Part 2: Study Description
Part 3: Data Files Description
Part 4: Variable Description
Part 5: Other Study-Related Materials
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Replication Data for: Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization

doi:10.21979/N9/EX7HL5

DR-NTU (Data)

2021-06-10

1

Miao, Yansong, 2021, "Replication Data for: Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization", https://doi.org/10.21979/N9/EX7HL5, DR-NTU (Data), V1, UNF:6:OO68jhscRg2tV3QwNHf5Bg== [fileUNF]

Replication Data for: Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization

doi:10.21979/N9/EX7HL5

Miao, Yansong (Nanyang Technological University)

Image J

Graphpad

start-up grant M4081533

Tier 2 MOE2016-T2-1-005S

Tier 1 RG38/17-S

SIG18002/M4062479

Tier 2 MOE2015-T2-1-078

DR-NTU (Data)

Miao, Yansong

Miao, Yansong

2020-03-31

https://doi.org/10.21979/N9/EX7HL5

Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Actin

published and original data for the publication

images

gel

10.1038/s41467-019-13125-1

Xie, Y., Sun, J., Han, X., Turšić-Wunder, A., Toh, J. D., Hong, W., ... & Miao, Y. (2019). Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization. Nature communications, 10(1), 1-18.

• Number of cases: 60

• No. of variables per record: 24

• Type of File: text/tab-separated-values

UNF:6:OO68jhscRg2tV3QwNHf5Bg==

• cytosolautoflurescencesignal(n=34) - cytosolautoflurescencesignal(n=34)
• B - B
• Aip5-GFP(n=41) - Aip5-GFP(n=41)
• D - D
• E - E
• F - F
• spa2∆Aip5-GFP(n=23) - spa2∆Aip5-GFP(n=23)
• H - H
• I - I
• J - J
• bni1∆Aip5-GFP(n=29) - bni1∆Aip5-GFP(n=29)
• L - L
• M - M
• N - N
• bud6∆Aip5-GFP(n=59) - bud6∆Aip5-GFP(n=59)
• P - P
• Q - Q
• R - R
• ratio(R)=(budtipsignal-autoflurescencesignalincytosol)/(cytosolsignal-autoflurescencesignalincytosol) - ratio(R)=(budtipsignal-autoflurescencesignalincytosol)/(cytosolsignal-autoflurescencesignalincytosol)
• T - T
• U - U
• V - V
• W - W
• X - X

f22262 Location:

Summary Statistics: StDev 11.696760097059617; Max. 218.3; Valid 35.0; Min. 173.514; Mean 195.43476470588234;

Variable Format: numeric

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f22262 Location:

Summary Statistics: StDev NaN; Max. NaN; Min. NaN; Mean NaN; Valid 0.0

Variable Format: numeric

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f22262 Location:

Variable Format: character

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Variable Format: character

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f22262 Location:

Summary Statistics: Min. NaN; Mean NaN; Max. NaN; Valid 0.0; StDev NaN;

Variable Format: numeric

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f22262 Location:

Variable Format: character

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f22262 Location:

Variable Format: character

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f22262 Location:

Summary Statistics: Mean NaN; Valid 0.0; Min. NaN; Max. NaN; StDev NaN;

Variable Format: numeric

Notes: UNF:6:ELKmaIjFilSyd/4uaPtuhw==

f22262 Location:

Variable Format: character

Notes: UNF:6:oxu6IF+9uLoVI76d/eN/pw==

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Variable Format: character

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f22262 Location:

Summary Statistics: StDev NaN; Mean NaN; Min. NaN; Max. NaN; Valid 0.0

Variable Format: numeric

Notes: UNF:6:ELKmaIjFilSyd/4uaPtuhw==

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Variable Format: character

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f22262 Location:

Summary Statistics: Valid 0.0; Min. NaN; StDev NaN; Max. NaN; Mean NaN;

Variable Format: numeric

Notes: UNF:6:ELKmaIjFilSyd/4uaPtuhw==

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Variable Format: character

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f22262 Location:

Summary Statistics: StDev NaN; Min. NaN; Mean NaN; Max. NaN; Valid 0.0;

Variable Format: numeric

Notes: UNF:6:ELKmaIjFilSyd/4uaPtuhw==

f22262 Location:

Variable Format: character

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Label:

03012018 Bni1-X sds ge figure-1.tif

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10uM Spa2-1151 Aip5 Montage figures.tif

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14082017 RGB Montage Aip5-G.tif

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1-Stack Aip6 spa2 no LatA.tif (RGB).tif

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1-Stack (RGB) no LatA.tif

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20uM Spa2-1151 Aip5 Figures Montage.tif

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21.31.45 CCD Acquire droplet spa2-1151 500nm scale bar.tif

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25degree GROWTH 14102017013.tif

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29062019spa2mutannts spotting stress figure.tif

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2-Stack no LatA(RGB).tif

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2-Stack (RGB) no LatA.tif

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30uM Spa2 1151 Aip5 Montage FIGURE.tif

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37degree GROWTH 14102017013.tif

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400 pixel figure Stack ph4.4-6.tif

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400 pixel figure Stack ph6.6-7.5 scale bar 5um.tif

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40uM Spa2-1151 Aip5 Montage figure.tif

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5%.tif

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5um scale bar montage figure spa2null Aip5-G.tif

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8c.tif

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actin.tif

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Aip5-1372 0.5uM 13122016-1.tif

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Aip5-1372 -1 new.tif

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Aip5-1372-1 .tif

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Aip5-1372-2 .tif

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Aip5-1372-3 .tif

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Aip5-1372 .tif

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Aip5-3072-1 new.tif

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Aip5-3072-1.tif

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Aip5-3072-2.tif

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Aip5-3072-3.tif

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Aip5-3072 Sum 12062016-2.tif

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Aip5-3072.tif

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Aip5-813-1 new.tif

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Aip5-813-1.tif

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Aip5-813-2.tif

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Aip5-813-3.tif

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Aip5-813 summary Dec06 2016-1.tif

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Aip5-813.tif

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Aip5-fl 2.5uM+Bni1 500nM orig.Stack.tif

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Aip5-fl 2.5uM orig. Stack.tif

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Aip5-fl 2uM cropped Stack-2.tif

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Aip5-fl 2uM+MBP-Bni1 300nM cropped Stack-2.tif

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Aip5-fl.jpg

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Aip5-G ED1h.tif

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Aip5-G.tif

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Aip5-G.tif

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aip5-mcherry RGB.tif

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Aip5-N 0.5uM 13122016-1.tif

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Aip5-N-1 new.tif

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Aip5-N-1.tif

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Aip5-N-2.tif

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Aip5-N-3.tif

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Aip5-N.tif

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Aip5-R Spa2-G 3um.tif

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cropped PS Merge rgb Stack-5 Bni1 Bud6.tif (RGB).tif

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DOT BLOT bni1 bnr1 25degree-1.tif

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E LatA RGB Montage aip5-GFP neck 25 50 neck.tif

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E LatA RGB Montage aip5-GFP neck.tif

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E LatA RGB Montage aip5-GFP.tif

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E RGB montage spa2 null bni null Aip5-GFP LatA.tif

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E RGB spa2 null aip5-GFP LatA neck.tif

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E RGB spa2 null aip5-GFP LatA neck.tif

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E RGB spa2 null aip5-GFP LatA tip.tif

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E scale bar 3um RGB 1-Montage bni1 null aip5-GFP LatA neck.tif

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E scale bar 3um RGB 1-Montage bni1 null aip5-GFP LatA.tif

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Fig1c.tif

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Fig2C.tif

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Fig2k.tif

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FIgure 4g.tif

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Figure montage WT EP1h glucose 2 5min.tif

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Figure S1g S7a.tif

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FIGURES Composite4 40uM spa2-1151 500nM Aip5G 10%PEG-1.tif

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for figuresComposite5 10uM spa2-1151 500nM Aip5G 10%PEG-1.tif

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Label:

FOR FIGURES Composite6 30uM spa2-1151 500nM Aip5G 10%PEG-1.tif

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for figuresComposite9 20uM spa2-1151 500nM Aip5G 10%PEG-1.tif

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Label:

GFP Stack-5 Bni1 Bud6-1.tif

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Image 13-1.tif

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Image 15-1.tif

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Image 16-1 gd.tif

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Image 27 example one Aip5fl peg.tif

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MBP-Bni1 300nM cropped Stack-2.tif

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MBP-Bni1 500nM orig.Stack.tif

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monomer Aip5-C.tif

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montage 10nMscBni1fh1c 5-10min.tif

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Montage 10%.tif

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Montage1-2um.tif

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Montage1-2um.tif

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Montage 1min gap.tif

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Montage 1% Scale bar 10um.tif

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Montage1.tif

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Montage1.tif

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Montage1.tif

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montage 20nMAip5 5-10min1.tif

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montage 20nMscAip5c+10nMscBni1fh1c 5-10min 5um.tif

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montage 20nM scAip5c 5-10min.tif

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montage 2.5%peg 1uM AIp5 0-12h-24h-36h.tif

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Montage 2and a half.tif

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Montage 2um.tif

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Montage 5-10min Actin.tif

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Montage 5um figure.tif

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Montage 5um.tif

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Montage 6a.tif

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Montage 7and a half.tif

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montage aip5-813-G s-m-L scale bar 3um .tif

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Montage aip5null figures2 .tif

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Montage aip5null.tif

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Montage bni1null for figures2 .tif

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Montage bud6 null aip5-GFP 1a.tif

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montage figure5c 100nm Aip5 PEG for figure5 54cm 200nm-1.tif

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Montage figure 6 scale bar 3um.tif

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Montage figure Aip5-G ED1h.tif

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Montage figure Aip5-G.tif

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Montage figure spa2null Aip5-G 5um.tif

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montage figure spa2null ED1h glucose 2 5-5um.tif

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Montage for figures 300 pixel.tif

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montage new 20042019 3um.tif

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Montage RGB 3um.tif

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Montage RGB 5um.tif

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Montage RGB Aip5-G ph5.5.tif

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Montage RGB .tif

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Montage RGB.tif

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Montage spa2 Ctrun-GFP 1a.tif

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Montage spa2null Aip5-G.tif

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Montage spa2null.tif

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Montage.tif

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Montage.tif

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Montage.tif

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Montage WT.tif

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no latA Aip5-G-1.tif

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no LatA Aip5-G.tif

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no LatA Bni1.tif

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no lata bud6-1.tif

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PS Merge rgb Stack-5 Bni1 Bud6.tif (RGB) scale bar 3um.tif

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purch.Actin 1.5uM orig. Stack.tif

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Reslice of Image-1.tif

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Reslice of Image-1.tif

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Reslice of Image-1.tif

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Reslice of Image-1.tif

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Reslice of Image-1.tif

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Reslice of Image-2.tif

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RFP Stack-5 Bni1 Bud6-2.tif

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rgb 5nM bni1fh1c 5-10min1.tif

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rgb actin 5-10min.tif

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RGB Aip5-FL+arginine.tif

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RGB bni1-12 bnr1null max-1.tif

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RGB bni1 null aip5-GFP 1a.tif

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RGB for figures montage aip5-c-GFP s-m-n.tif

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RGB Montage aip5 Ctrun-GFP.tif

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RGB Montage aip5-GFP 1a.tif

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RGB Montage aip5null bni1null figure2.tif

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RGB montage WT figure2.tif

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rgb scAip5+bni1fh1c 5-10min rgb 5um.tif

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RGB spa2 Ctrun aip5-3mCherry 1a.tif

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RGB spa2 null aip5-GFP 1a.tif

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S5b.tif

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S5h-1.tif

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S5h.tif

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S6c.tif

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S6d.tif

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S7d.tif

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scale bar 3um bni1-12 bnr1null aip5null abp140-1.tif

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Spa2-G 3um.tif

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spa2-G RGB.tif

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spa2null Aip5-G ED1h.tif

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Stack 100nM Aip5-G 20%peg10000-1.tif

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Stack1 ph6.5.tif

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Stack1.tif

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Stack1.tif

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Stack1.tif

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Stack1.tif

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Stack 30s gap Aip5-GFP.tif

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Stack 500nM spa2-LC-1.tif

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Stack Aip5g 10%peg 1min interval.tif

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Stack Aip5-G 30s gap.tif

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Stack Aip5-G 30s gap.tif

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Stack Aip5-GFP ph5.5 10s gap.tif

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Stack Aip5-GFP sp2null 1min interval.tif

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Stack Aip6 spa2 no LatA.tif (RGB).tif

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Stack no LatA(RGB).tif

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Stack spa2null Aip5-G ph5.5 10s gap.tif

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WB S1D AIp5-GFP anti-GFP wet.tif

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WB S1D normal AIp5-GFP pgk1-wet.tif

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