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Part 1: Document Description
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Citation |
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Title: |
Replication Data for: Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress |
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Identification Number: |
doi:10.21979/N9/OLY1FU |
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Distributor: |
DR-NTU (Data) |
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Date of Distribution: |
2020-05-27 |
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Version: |
1 |
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Bibliographic Citation: |
Koh, Jhee Hong; Lei, Wang; Beaudoin-Chabot, Caroline; Thibault, Guillaume, 2020, "Replication Data for: Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress", https://doi.org/10.21979/N9/OLY1FU, DR-NTU (Data), V1 |
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Citation |
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Title: |
Replication Data for: Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress |
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Identification Number: |
doi:10.21979/N9/OLY1FU |
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Authoring Entity: |
Koh, Jhee Hong (Nanyang Technological University) |
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Lei, Wang (Nanyang Technological University) |
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Beaudoin-Chabot, Caroline (Nanyang Technological University) |
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Thibault, Guillaume (Nanyang Technological University) |
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Software used in Production: |
Microsoft Excel |
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Software used in Production: |
Adobe Illustrator |
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Software used in Production: |
Adobe Photoshop |
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Software used in Production: |
Graphpad Prism |
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Software used in Production: |
ZEN Black |
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Grant Number: |
Academic Research Fund Tier 1 2016-T1-001-078 |
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Distributor: |
DR-NTU (Data) |
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Access Authority: |
Thibault, Guillaume |
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Depositor: |
Koh Jhee Hong |
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Date of Deposit: |
2019-04-29 |
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Holdings Information: |
https://doi.org/10.21979/N9/OLY1FU |
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Study Scope |
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Keywords: |
Medicine, Health and Life Sciences, Medicine, Health and Life Sciences, Endoplasmic reticulum, ER, Unfolded protein response, UPR, Lipid bilayer stress, Autophagy, Metabolic disease |
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Abstract: |
Metabolic disorders, such as non-alcoholic fatty liver disease (NAFLD), are emerging as epidemics that affect the global population. One facet of these disorders is attributed to the disturbance of membrane lipid composition. Perturbation of endoplasmic reticulum (ER) homeostasis through alteration in membrane phospholipids activates the unfolded protein response (UPR) and causes dramatic transcriptional and translational changes in the cell. To restore cellular homeostasis, the three highly conserved UPR transducers ATF6, IRE1 (also known as ERN1 in mammals) and PERK (also known as EIF2AK3 in mammals) mediate adaptive responses upon ER stress. The homeostatic UPR cascade is well characterised under conditions of proteotoxic stress, but much less so under lipid bilayer stress-induced UPR. Here, we show that disrupted phosphatidylcholine (PC) synthesis in Caenorhabditis elegans causes lipid bilayer stress, lipid droplet accumulation and ER stress induction. Transcriptional profiling of PC-deficient worms revealed a unique subset of genes regulated in a UPR-dependent manner that is independent from proteotoxic stress. Among these, we show that autophagy is modulated through the conserved IRE-1–XBP-1 axis, strongly suggesting of the importance of autophagy in maintaining cellular homeostasis during the lipid bilayer stress-induced UPR. |
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Kind of Data: |
Experimental data |
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Methodology and Processing |
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Sources Statement |
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Data Access |
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Other Study Description Materials |
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Related Publications |
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Citation |
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Identification Number: |
10.1242/jcs.217992 |
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Bibliographic Citation: |
Koh, J. H., Wang, L., Beaudoin-Chabot, C., & Thibault, G. (2018). Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress. J Cell Sci, 131(22), jcs217992-. |
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Citation |
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Identification Number: |
10356/89666 |
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Bibliographic Citation: |
Koh, J. H., Wang, L., Beaudoin-Chabot, C., & Thibault, G. (2018). Lipid bilayer stress-activated IRE-1 modulates autophagy during endoplasmic reticulum stress. Journal of Cell Science, 131(22), jcs217992-. |
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Figure S1.zip |
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